Part:BBa_M33355

pTod + Purple Color Generator

Pseudomonas putida possesses a two-component regulatory system (TCS) which can sense changes in the toluene level of the environment. TodS and TodT proteins control the expression of genes involved in the degradation of toluene, benzene, and ethylbenzene via the toluene dioxygenase pathway. The catabolic genes of the toluene dioxygenase pathway are transcribed from a single promoter termed pTod, located upstream from the todX gene. The TodS/TodT TCS mediate pTod in response to toluene and other aromatic hydrocarbons. Once TodT is activated, it binds to a site on the pTod promoter approximately 107 base pairs upstream from the RNA polymerase-binding site. DNA bending caused by integration host factor (IHF) enables contact between TodT and RNA polymerase, initiating transcription.

This part should be used in conjunction with BBa_M33350, the CDS for TodS downstream of an RBS, and BBa_M33354, the CDS for TodT downstream of an RBS.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1153
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 5726
  Illegal BamHI site found at 4413
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 3938
  Illegal AgeI site found at 4134
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 6676
  Illegal SapI.rc site found at 6751